广东省人民医院/广东省医学科学院林蒋国实验室的研究工作主要聚焦于凝血止血、炎症反应及肿瘤转移等病生理过程中的重要科学问题，从分子、细胞、动物等多维度多层次研究其中核酸、蛋白和细胞功能，旨在发现其中的调控机制，寻找新的生物标记物及开发新药物。实验室负责人林蒋国研究员于华南理工大学获得工学学士和博士学位。曾在美国乔治亚理工学院进行博士联合培养、美国北卡罗来纳州立大学进行博士后训练。2015年作为高层次人才被引进到华南理工大学生物科学与工程系；2020年作为高层次人才被引进到广东省人民医院医学研究部。本实验室先后主持国家自然科学基金、广东省自然科学基金、广州市科技计划等项目7项。在Blood、Nucleic Acids Res、Protein&Cell、Thromb Res等高水平杂志上发表论文20多篇。

实验室成员介绍：

林蒋国   方 翔  方金花  吴芝伟  杨俊贤  彭智童  

实验室学术亮点：

1） 建立了从原子水平到动物水平的生物大分子互作和功能研究的实验技术和分析方法，为生物大分子互作机制和药物开发提供了新见解和新思路；

2） 利用原子力显微镜系统、高分辨荧光流动腔系统以及磁镊系统模拟不同血流和组织硬度等力学微环境下，生物大分子互作和细胞功能的改变，揭示病生理过程中的力学生物学调控机制。

代表性论文：

1. Lin J, Ding X, Yang P, Liu S, Li Q, Cruz M A, Dong J-F, Fang Y, Wu J. Force-induced biphasic regulation of VWF cleavage by ADAMTS13. Thromb Res. 2023 229:99-106

2. Wu Z, Yang J, Xie X, Liu G, Fang Y, Wu J, Lin J. Residues R1075, D1090, and C1130 Are Critical in ADAMTS13 TSP8-Spacer Interaction Predicted by Molecular Dynamics Simulation. Molecules. 2021 26(24):7525

3. Fang X, Gamallat Y, Chen Z, Mai H, Zhou P, Sun C, Li X, Li H, Zheng S, Liao C, Yang M, Li Y, Yang Z, Ma C, Han D, Zuo L, Xu W, Hu H, Sun L, Li N. Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development. 2021 Jun 1;148(11):dev199216.

4. Yang J#, Wu Z, Xie X, Liu G, Fang Y, Wu J, Lin J*. Characterization of the interactions of ADAMTS13 CUB1 domain to WT- and GOF-Spacer domain by molecular dynamics simulation. J Mol Graph Model. 2021 Dec;109

5. Fang J, Sun X, Liu S, Yang P, Lin J, Feng J, Cruz MA, Dong JF, Fang Y*, Wu J*. Shear Stress Accumulation Enhances von Willebrand Factor-Induced Platelet P-Selectin Translocation in a PI3K/Akt Pathway-Dependent Manner. Front Cell Dev Biol. 2021 Jun 1;9.

6. Yang J#, Wu Z, Long Q, Huang J, Hong T, Liu W, Lin J*. Insights Into Immunothrombosis: The Interplay Among Neutrophil Extracellular Trap, von Willebrand Factor, and ADAMTS13. Front Immunol. 2020 Dec 2;11:

7. Chen X#, Dai J#, Lin J#, Wu Y#, Ouyang J, Huang M, Zhuang J, Fang Y*, Wu J*. Image-based morphometric studies of human coronary artery bifurcations with/without coronary artery disease. Comput Methods Biomech Biomed Engin. 2020 Nov 30:1-17.

8. Yu S#, Liu W, Fang J, Shi X, Wu J, Fang Y*, Lin J*. AFM Imaging Reveals Multiple Conformational States of ADAMTS13. J Biol Eng. 2019 Jan 22;13:9.  

9. Li Zhenhai#; Lin Jiangguo#; Sulchek Todd; Cruz Miguel A; Wu Jianhua; Dong Jing Fei; Zhu Cheng*; Domain specific mechanical modulation of VWF-ADAMTS13 interaction., Molecular Biology of the Cell, 2019, 30(15):1920-1929.

10. Fang X, Huang LL, Xu J, Ma CQ, Chen ZH, Zhang Z, Liao CH, Zheng SX, Huang P, Xu WM, Li N, Sun L. Proteomics and single-cell RNA analysis of Akap4-knockout mice model confirm indispensable role of Akap4 in spermatogenesis. Dev Biol. 2019 Oct 15;454(2):118-127.

11. Fang X#, Lin J#, Fang Y*, Wu J*. Prediction of spacer-α6 complex: a novel insight into binding of ADAMTS13 with A2 domain of von Willebrand factor under forces. Sci Rep. 2018 Apr 10;8(1):5791.

12. Lin J#, Countryman P, Chen H, Pan H, Fan Y, Jiang Y, Kaur P, Miao W, Gurgel G, You C, Piehler J, Kad NM, Riehn R, Opresko PL, Smith S, Tao YJ, Wang H*. Functional interplay between SA1 and TRF1 in telomeric DNA binding and DNA-DNA pairing. Nucleic Acids Res. 2016 Jul 27;44(13):6363-76.  

13. Lin J#, Kaur P, Countryman P, Opresko PL, Wang H*. Unraveling secrets of telomeres: one molecule at a time. DNA Repair (Amst). 2014 Aug; 20:142-153.

14. Lin J#, Countryman P, Buncher N, Kaur P, E L, Zhang Y, Gibson G, You C, Watkins SC, Piehler J, Opresko PL, Kad NM, Wang H*. TRF1 and TRF2 use different mechanisms to find telomeric DNA but share a novel mechanism to search for protein partners at telomeres. Nucleic Acids Res. 2014 Feb;42(4):2493-504.

15. Wu T#, Lin J#, Cruz MA, Dong JF, Zhu C*. Force-induced cleavage of single VWFA1A2A3 tridomains by ADAMTS-13. Blood. 2010 Jan 14;115(2):370-8.

实验技术亮点：

1、高分辨荧光流动腔

2、原子力显微镜

3、磁镊计术

实验团队照片